Approaches in Lifelong Learning free essay sample

The need of the learners was identified as having to have investigators who could adopt a methodical approach, which is essential whilst carrying out equipment failure investigations. This was used as my session aim: ‘This learning session will teach you a methodical approach of investigating equipment failure’. When identifying the need for training I first assessed what the learners would have to have learnt by the end of the learning session. This was identified as the need to know what equipment would be used and how to carryout an investigation in easily managed phases. I then used this as my objective: By the end of this lesson you will have been introduced to the contents of the investigator pack and be able to identify the 3 phases of an equipment failure investigation’. (1. 2) The learners were identified as personnel with some previous learning within the subject matter. The instructional, rather than practical approach to delivering the session was selected as the subject matter was mainly theoretical (the session would later be followed by a practical session). We will write a custom essay sample on Approaches in Lifelong Learning or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page The primary method of delivery would be verbal, given by the teacher.

Answers to Questions About Articles

Answers to Questions About Articles Answers to Questions About Articles Answers to Questions About Articles By Mark Nichol 1. I found the follow information about the indefinite article a in The Oxford Advanced Learner’s Dictionary: â€Å"used before uncountable nouns when these have an adjective in front of them, or phrase following them. For example, â€Å"a good knowledge of French†; â€Å"a sadness that won’t go away.† I dont understand the information. The dictionary says that the a is used before uncountable nouns when these have an adjective in front of them. But, as far as I know, the indefinite article a cannot be used in front of uncountable nouns. Does the information mean that we can always use the indefinite article a in front of uncountable nouns that have an adjective in front of them? Is it a rule? Mass, or noncount, nouns can be preceded by the indefinite article a when they are modified by a preceding adjective or a subsequent phrase: For example, though you would refer to bravery as courage, not â€Å"a courage,† you can write of â€Å"an uncommon courage† and â€Å"a courage like no other.† However, the passage from the resource you mentioned refers only to the possibility of the former type of usage, not to its ubiquity; it is rare. 2. Something I would like some clarification on is the use of a or an before the word holistic. I have been taught an, but this doesn’t seem to make sense to me, as there are many instances when a word beginning with h is preceded by a rather than an. Are you able to shed some light on this? Use a or an before a word that begins with the letter h depending on whether the h is pronounced: â€Å"a historic occasion,† but â€Å"an honest mistake.† 3. I don’t know what to do with the names of institutions when they call themselves a name with the in the title for example, â€Å"the Open Door.† In the middle of a sentence, do you have to capitalize the? Would you say, â€Å"We met at The Open Door†? The direct article should be lowercase even when it is integral to an entity’s name (as in â€Å"the American Automobile Association,† when it would not be referred to, minus the, as American Automobile Association†), but many entities insist on capitalizing it as part of a branding identity. (And it’s best to do so for indirect articles, as in, for example, the name of a community center called A Place for Teens.) If you work for the Open Door or it’s giving your organization money or other consideration and management at the Open Door wants the name treated as â€Å"The Open Door,† treat it as â€Å"The Open Door.† Otherwise, style it â€Å"the Open Door.† Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Grammar category, check our popular posts, or choose a related post below:Useful Stock Phrases for Your Business EmailsRules for Capitalization in TitlesComment, Suggestion, and Feedback

Rhetorical Devices Essay Example | Topics and Well Written Essays - 250 words

Rhetorical Devices - Essay Example This is a very complex and complicated matter. Now this  resolution will pass, although we all know that the President can wage a war even without it. However difficult this vote may be, some of us must urge the use of restraint. Our country is in a state of mourning. Some of us must say, lets step back for a moment. Lets just pause, just for a minute and think through the implications of our actions today, so that this does not spiral out of control. Now I have agonized over this vote. But I came to grips with it today, and I came to grips with opposing this resolution during the very painful, yet very beautiful memorial service. As a member of the clergy so eloquently said, "As we act, let us not become the evil that we deplore." The speech of Barbara Lee in the House of Representatives on September 14, 2001 was a speech concerning H. J. Res. 64, or the authorization for the use of military force against those responsible for the 9-11 terrorist attacks in the United States. It was obvious from the speech that she did not want to vote for the passing of the bill because of her moral stance against violence and vengeance. In her speech, she showed her opposition to the proposed military authorization using certain rhetorical devices. For her logos or the use of reason in order to achieve her goal of convincing her fellow representatives of the futility of the military authorization, she used the statement: â€Å"†¦I am convinced that military action will not prevent further acts of international terrorism against the United States. This is a very complex and complicated matter† (Lee, 2001). This rhetorical device is credible. It is actually meant to give a logical reason to the whole speech, specifically to why the speaker refuses the military authorization. The idea of terrorism is indeed more difficult and complicated than something that can just be quelled by

Evidence Based Practice - Vascular Bleeding Following PCI Essay

Evidence Based Practice - Vascular Bleeding Following PCI - Essay Example 3.0%), particularly hematoma (1.1% vs. 2.5%) and pseudoaneurysm (0.1% vs. 1.0%), as compared to MC use (n = 951). Odds ratio of developing complications for VCD recipients was 0.56 (95% CI: 0.40 – 0.79). Because baseline characteristics between treatment groups were initially different, statistical adjustment was done. In 2010, a prospective, small-scale, short-term case-series study involving 413 patients treated of acute coronary syndrome was used to identify predictors of vascular complications. The use of VCDs by the sample was fairly limited, only 62 used Angio-Seal and 19 had PerClose. For those who used VCDs, there were no significant differences in the occurrence of hematoma during or after the use of procedure. In addition, those with complications were old and had significantly higher systolic blood pressure, as compared to those that did not have complications (Sulzbach-Hoke, Ratcliffe, Kimmel, Kolansky, & Polomano, 2010). The different VCDs were also compared. In patients that have received PCI in the past eight years, those who underwent VCD (n = 2, 814) had significantly lower incidence rate of ≠¥ 10 cm hematoma as compared to those who were given MC (n = 1, 407), 1.1% vs. 2.1%. Baseline characteristics between VCD and MC groups were not statistically different. Comparison among Angio-Seal, Perclose and Starclose users revealed that those who used Perclose had the least rate of complications (5.6%), while those receiving Starclose had the most (10.2%). The differences among rates were significant (Iqtidar, Li, Mather, & McKay, 2011). A similar, wider scale, retrospective study (n = 1, 819, 611) was conducted 2012 by Tavris, et al. The rates of bleeding in Angio-Seal (28.7%), Perclose (8.3%), Boomerang Closure Wire (BCW) (0.6%) and hemostasis patches (7.9%) VCDs were less than 1%, while those receiving MC (38.9%) was 1.18%. In contrast, StarClose (4.4%) and Mynx

Critical Evaluation of HRM and Organisation Behaviour Theories Coursework - 2

Critical Evaluation of HRM and Organisation Behaviour Theories Frameworks - Coursework Example The paper explains that human resource management is a broad spectrum of professional mandate that has to do with anything that deals with the handling of people (human resource). As far as the people are concerned, human resource management caters for â€Å"compensation, hiring, performance management, organization development, safety, wellness, benefits, employee motivation, communication, administration, and training†. Human resource management has been given a theoretical framework by the Business Ball whereby it is related to the psychological contract. It is said that â€Å"The Psychological Contract' is an increasingly relevant aspect of workplace relationships and wider human behavior.† This means that human resource management is considered a necessary phenomenon not just because of the fact that it is needed to lead to the growth and development of an organization but then because it is needed to be in place to protect the basic relationship that needs to exis t between the human workforces in the organization.   This makes human resource management an aspect of organizational management that is needed to have a more permanent basis. It is in this regard that human resource management has always been discussed along with the lines of organizational behavior – because the organizational behavior has a more permanent stake in all organizations. Organizational Behavior has been explained as â€Å"the study and application of knowledge about how people, individuals, and groups act in organizations† (Clark, 1998). This means that organizational behavior can lead to a better comprehension of the type of human resource management that should be in place at a workplace or a given organization and this is the reason why the two concepts are always treated hand in hand. Assumptions underpinning the way in which HRM and organizational behavior theories and frameworks have been propagated Rank (2011) notes that human resource management and organizational behavior has existed with humankind since time immemorial. The only difference is that these two phenomena of management have been through a lot of changes and transitions. The good news is that handling of both human resource management and organizational behavior has been improving with time. There is indeed exists a chain of assumptions underpinning why and how theories and framework of human resource management and organizational behavior all came about. Rank (2011) attributes the conception of the two theories and framework to the emancipation of human rights such that the need to promote the well-being and welfare of all people - regardless of whether they are employees or employers was deemed necessary.  

The Purpose Of Punishment In The Justice System Criminology Essay

The Purpose Of Punishment In The Justice System Criminology Essay Case Study 2: The purpose of Punishment within the criminal justice system. What role does punishment serve within the criminal justice system? Consider the part played by reductivism, incapacitation, retribution, deterrence and rehabilitation as by-products of the perceived need for the criminal justice system to punish offenders. How have political policies and other ideologies affected the state emphasis on the need to punish offenders? In the following paragraphs it is going to be explained the role of punishment within the criminal justice system. Furthermore, we are going to get involved with theories and objectives related to punishment such as: reductivism, deterrence, rehabilitation, incapacitation and finally retribution. Beginning our case study we have to argue that there is considerable controversy over the effectiveness of punishment in reducing crime, but whether or not its actual effectiveness, still is the only method being used conforming someone who made a misdeed, back to society and freedom. Punishment can be simply defined as a legally approved method designed to facilitate the task of crime control (Carrabine, 2004), and its main purpose is to rehabilitate the offender, expiate the victim and dissuade others from becoming wrongdoers. In order to successfully create a holistic view on the matter, we have to focus on many punishment perspectives and theories as it is the only way for a critical evaluation. punishment as a social institution is an inherently complex business that needs to be approached from a range of theoretical perspectives as no single interpretation will grasp the diverse meanings generated by punishment (Carrabine, 2004). The reductive theory of punishment justifies that punishment occurs because it helps to prevent and reduce future consequences of crime, acting as a forward-looking theory for the general good. Moreover, claims that if punishment takes place, future crime will be less than if no penalty were inflicted. For punishment to reduce future crimes, the pain and unhappiness caused to the offender must be outweighed by the avoidance of unpleasantness to other people in the future (Cavadino, 2002). Therefore, it is a moral action against criminals (famously advanced by Jeremy Bentham 1748-1832) since it produces the greatest happiness of the greatest number of people. Nevertheless, there are many mechanisms of reduction which will be shown below. Deterrence based on utilitarian theories, is a method of reduction and its main perspective is that if you cause someones fear, then he will be afraid to offend and break the law (tough on crime). Moreover, separates deterrence into general and individual justifying that general is when punishment dissuades others from following the offenders example, in spite of individual deterrence which aims to teach delinquent not to repeat the behaviour. Deterrence lacks to produce strong and validate evident of its effectiveness as no certain penalty prevented someone from committing a given crime. What is more, referring to the individual deterrence, my opinion is that we cannot say whether or not an offender stopped his criminal behaviour, simply because not all crimes are being convicted. Also, according to official crime statistics, I believe, there is not such effectiveness as Michael Howards supported about prison/punishment and toughness on crime. Prison works. It ensures that we are protected from murderers, muggers and rapists- and it makes many who are tempted to commit crime think twice (Michael Howard, Home Secretary 1993). Rehabilitation is another mechanism of crime reduction which is going to be produced and evaluated on the following paragraphs. According to rehabilitation the idea of punishment is to apply treatment to the offender so that he is made capable afterwards to return back to the society as a law-abiding member. Rehabilitation is viewed as a humane alternative comparing to the harshness of retribution and deterrence giving more a programme function to the punishment, without that meaning that an offender would receive a more lenient penalty for his offence. An important feature of rehabilitation is that the offender could stay on probation whether that means outside prison or inside until he is thought to be ready. Critics though assume that if the prison administrator is the responsible one who decides if offender made a progress and he is ready to go, then corruption may occur which will falsify the true situation. Finally, another issue is that an offender guilty for minor crime proba bly could not tolerate lengthy detentions simple because of inability or refusal to adopt a subservient attitude toward prison officers and authority in general. Continuing our study we will refer to retributive theory which is in total antithesis of reductivism and justifies that punishment came as result of criminality. An attractive feature is that retributivism is a natural connection between the retributive approach and the idea that both offenders and victims have rights. Reductivist theory always founds it difficult to encompass the notion of rights, even when it comes to providing entirely innocent people with a right not to be punished. Retributivism has no such problem, since it follows automatically from the retributive principle that it must be wrong to punish non-offenders. Criminals, according to retributivist principle deserve the punishment because in some way, evil for evil somehow make a right. Additionally, according to retributivism, severity of a punishment should be proportionate to the gravity of the offence (tariff). What is more, retributive punishment, argues that applies fairly and equally to all of us as long as we all live in the same equilibrium followed by the same norms and values. The main issue with such a theory is that it would be objective only if we were all genuinely equal sharing the same advantages. Detected offenders typically start from a position of social disadvantage (Cavadino, 2002). From the moment retributive punishment tries to inflict equality restoring the balance, then increases inequality rather than do the opposite. The last theory refers to the act of making the offender not capable of committing a crime and is known as the incapacitation theory. According to that, offenders who have committed repeated crimes or thought to be dangerous are being punished by execution or lengthy incarceration (life imprisonment). Such a punishment though unfortunately makes it difficult to identify that kind of offenders. Thus, it is extremely controversial the principle of incapacitation especially to those who assist that punishment should advocate equal retribution followed by dignity. An important controversial example evaluating incapacitation is the chemical-castration of sex offenders (against children) with hormonal drugs which was first adopted in the U.S of California in 1996 and proved that drugs alone did not make the offender incapable of committing sex crimes. To conclude, I would like to say that I have not come to a conclusion yet on whether or not punishment actually helps and rehabilitates the delinquents. But I am sure that punishment so far is the function which separates those who live legally and those who do not. I also believe that the fear of punishment changes people behaviour a lot, as we all are afraid of punishment is that not true? Ultimately, I would like to finish with Sir Thomas Mores opinion which finds me totally agreed. Society first creates thieves, and then punishes them for stealing. There has always existed a curiously symbolic relationship between the criminal and society. It is not so much that society tolerates crime, rather the structure of modern society inevitably creates situations and circumstances in which crime occurs(Weisser, 1979).

A Worn Path, The Bean Trees, Little Women, How It Feels To Be Colored M

Courageous and Heroic Women in A Worn Path, The Bean Trees, Little Women, How It Feels To Be Colored Me, and the The Piano  Ã‚   Women have been heroic and courageous throughout history, though not all of these heroic (or courageous) adventures have been shown in any way.   Some of women’s heroic and courageous adventures are portrayed through short stories, books, and movies.   Eudora Welty’s short story â€Å"A Worn Path,† Barbara Kingsolver’s book The Bean Trees, Louisa May Alcott’s book Little Women, Zora Neale Hurston’s story â€Å"How It Feels To Be Colored Me,† and the movie â€Å"The Piano† all show women going through their own amazing and heroic adventures.   In these stories, the authors and director share with the reader (and watcher) the heroics of a woman.   Though these heroic adventures are full of risk and danger they are very valuable adventures for the characters’ health and development. In â€Å"A Worn Path† the character Phoenix Jackson walks through the forest on her way to town to get her grandson some medicine.   Now, Phoenix is an elderly lady, so this trip takes her awhile.   She has to go up and down hills, through thorny thickets, and over streams on logs.   Throughout the story she talks to herself and encourages herself to go on.   She talks to the brushes and the animals that she hears and tells them to keep â€Å"out of my way all you foxes, owls, beetles, jack rabbits, coons and wild animals!   Keep from under these feet†¦Ã¢â‚¬  (Gilbert and Gubar 1641). This trip shows how heroic Phoenix is, for it’s pretty risky for her to take.   It might not be as risky for a younger person, but it’s hard for an older woman to go through barbed-wire fences, over logs, and through ditches.   She could get caught or hurt on any of the obstacl... ...es where he gets to do more with her than she wants, like touching her, taking off some of her clothes, and eventually sleeping with her.     Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   All of these women show their courage in some way or another, whether that courage is of the heart, mind, or body.   Women have had heroic and courageous qualities throughout history, but these women are some very good examples of how some women have the nerve and bravery to do and think what they feel is right no matter what men or other women think of them.   Works Cited: Alcott, Louisa May. Little Women. New York: Signet, 1983. Hurston, Zora Neale. "How it Feels to be Colored Me." World Tomorrow, 11 (May, 1928) Kingsolver, Barbara. The Bean Trees. New York : Harper, 1988. Welty, Eudora. "A Worn Path." Perrine's Story & Structure. 9th ed. Ed. Thomas Arp. Tokyo: Harcourt Brace Jovanovich, 1997.

Endothelial tight junction proteins Essay

Endothelial tight junction proteins Introduction            The endothelium is situated at the inner side of all kinds of vessels and comprises of a monolayer of endothelial cells. Inter-endothelial junctions comprise junctional complexes, such as adherens junctions (AJ), tight junctions (TJ) and gap junctions (GJ) that play essential roles in tissue integrity, barrier function and intercellular communication respectively. These junctional complexes are related to those found at epithelial junctions with notable changes in terms of certain molecules and structure.            Endothelial junctional proteins play important roles in tissue integrity but also in vascular permeability, leukocyte extravasation and angiogenesis. Dormant endothelium may be exposed to stimuli provoking leukocyte extravasation at seditious sites and propagating angiogenesis. Both activities have an intense impact on endothelial cell-cell junctions.            Tight junctions aid the major functional objective of establishing a barrier inside the membrane, by controlling paracellular permeability and sustaining cell polarity. They achieve this by constricting apical or basolateral transmembrane diffusion of lipids and they have been suggested to contribute in regulating proliferation and differentiation of epithelial cells. However, the components that are involved and the signal routes concerned are unknown (Mitic & Anderson 1998).            Tight junctions are made up of integral membrane proteins claudins, occludin, tricellulin, junctional adhesion molecules (JAMs), including many peripheral membrane proteins such as the scaffold PDZ- domain proteins. This review will however, focus on ZO-1 and ZONAB. Histology of endothelia junctions            The junctional structures situated at the endothelial intercellular fissure are related to those located at the epithelium; however, their formation is more inconsistent and in most vascular beds their topology is less constrained than in epithelial cells. Adherens junctions, tight junctions and gap junctions are in most cases intermingled and create a complex zonular system with disparities in depth and thickness of the sub-membrane plate associated with the junctional structure (Franke et al. 1988; Rhodin 1974). In contrast to epithelial cells, GJs are often found close to the luminal surface. Hence, the term â€Å"Apical junction† used to jointly describe epithelial TJ and AJ may not be applied to the endothelium. The endothelium forms the vascular barrier with controlled permeability properties between the blood and the underlying tissues.            Tight junctions exhibit considerable inconsistency among different segments of the vascular tree (Franke et al. 1988). This disparity composes a major evidence of vascular bed differentiation of endothelial cells and has a strong impact on vascular permeability and leukocyte extravasation. Variations concern the complexity degree of the occluding strands as well as tight junction composition.            Large Artery endothelial cells, which are exposed to high flow rates, display a well-developed system of tight junctions. Within the microvasculature, tight junctions are less complex in capillaries than in arterioles, and even less in venules. It is important to mention that, post-capillary venules are the primary site of leukocyte extravasation, and accordingly, they display a high content of permeability mediator receptors, such as those for histamine, serotonin and bradykinin. On the other hand, blood brain barrier (BBB) and the blood retinal barrier (BRB) are predominantly rich in Tight Junctions and endothelial tight junctions have been principally studied in these sites.            Endothelial intercellular realms differ from those of epithelial cells by the absence of desmosomes (Franke et al. 1988). The transitional filaments, comprised in the endothelium by vimentin molecules, are poorly connected to cell-cell contacts. However, contrary to the situation in epithelia, the vimentin filaments may be associated to endothelial adherens junctions in junctional structures similar to desmosomes, called complexus adherens.            It must be emphasized that interendothelial junctions are vibrant structures, subjected to multiple regulations. Moreover, leukocytes extravasate majorly in postcapillary venules either through transcellular or paracellular methods. Extravasation via the intercellular junction is a rapid and controlled process, through which the leukocyte is squeezed in the fissure (diapedesis), followed by rapid junction reformation.            ZO-1 is a protein located on the cytoplasmic membrane plate of intercellular tight junctions and is engaged in transducing signals at cell-to-cell junctions. ZO-1 links tight junction transmembrane proteins to a cytoplasmic plaque and the actin-based cytoskeleton (Aijaz et al. 2006; Tsukita et al. 2001). In epithelial cells, ZO-1 interrelates with the transcription factor ZONAB to regulate cells proliferation in a cell density related manner (Balda & Matter 2000); however, the functions of ZO-1 and ZONAB in endothelial cells are still not clearly understood.            Unpublished work shows that downregulation of ZO-1 in endothelial cells stimulates redistribution of two transmembrane proteins; claudin-5 and JAM-A, and radical changes in the cytoskeleton affecting the localization of mechanosensor proteins and VE-cadherin role in the control of cell-cell tension.            These observations imply that one function of ZO-1 in endothelial cells is to coordinate components of the tight junction and associate them to the cortical cytoskeleton. However, it is unfamiliar whether the ZO-1 associated transcription factor ZONAB is linked to such ZO-1 effects.            Despite the fact that, ZO-1 explicitly associates with epithelial tight junctions (Stevenson et al. 1986), it has been observed that the protein appears in the nucleus in the process of proliferation (Gottardi et al. 1996). While the functional impact of the nuclear localization is currently not clear, studies reveal that these discrete subcellular distributions of ZO-1 are exquisitely sensitive to the state of cell-to-cell contact.            ZO-1 plays a major role of restraining ZONAB and regulates its accumulation in the nucleus through cytoplasmic sequestration. MDCK cells found in the epithelium exhibit two forms of this Y-box transcription factor (ZONAB) i.e. ZONAB -A and ZONAB -B which vary in a 68-amino acid supplement. Both categories of ZONAB bind to ZO-1 and link with intercellular junctions (Balda & Matter 2000).            ZONAB was initially designated in canine kidney epithelial cells (MDCK) and is a Y-box transcription factor. Y-box transcription factors are multipurpose control mechanisms of gene expression and studies suggest that they play a common role in enhancing proliferation (Bargou et al. 1997). ZONAB is one of the tight junction-associated dual localization protein: it localizes to junctions where it attaches to the SH3 surface of the adaptor protein ZO-1, and to the nucleus where it regulates transcription.            The distribution of ZONAB is controlled by the cell density as it localizes to both junctions and nuclei in low density, proliferating cells, and becomes constrained to the cytoplasm in high density cells (Balda & Matter, 2000). This distribution is also exhibited in its transcription activity, as ZONAB is transcriptionally vigorous in proliferating cells but inactive in non-proliferating cells. In the MDCK cells, ZONAB is necessary for normal rates of proliferation and controls G1/S phase transition (Balda et al. 2003).            ZONAB affects cell cycle development by two distinct processes: it controls the nuclear accumulation of CDK4 through a direct interaction and controls manifestation of genes encoding cell cycle regulators for example, PCNA and cyclin D1 (Balda et al. 2003; Sourisseau et al. 2006 ).            In 3D principles of MDCK cells, regular ZO-1 and ZONAB processes are necessary for epithelial cyst formation, implying that the Y-box transcription factor also controls epithelial differentiation (Sourisseau et al. 2006). Since ZO-1 and ZONAB can also relate with other types of intercellular junctions, for instance the gap junctions, in cells that lack tight junctions, it is possible that ZO-1 or ZONAB signaling is also of useful significance in other cell types other than epithelia (Ciolofan et al. 2006; Giepmans & Moolenaar 1998). Aims of the study            The aim of the study is to understand the functional consequences of downregulation of ZONAB in endothelial cells, and whether and how ZONAB cross-talks with other junctional components to regulate endothelial cell migration, proliferation and angiogenesis. Currently, we are looking at similarities and differences between the phenotype of downregulation of ZO-1 or ZONAB by RNA interference. Changes in expression and localization of a given protein are analysed using specific antibodies for immunoblots and immunofluorescence. Preliminary Results            It is observed that downregulation of ZO-1 or ZONAB resulted in similar redistribution of actin and vinculin from cell-cell junctions to stress fibers and focal adhesions, respectively. However, the localization of transmembrane proteins such as Claudin-5 and JAM-A is affected by downregulation of ZO-1 rather than by downregulation of ZONAB. The localization of the polarity protein PAR-3 is changed in both conditions.            Additionally, downregulation of ZONAB causes changes in ZO-1 by immunofluorescence that needs to be tested for expression by immunoblots. Next, we will characterize other transmembrane proteins (e.g. MD3 and claudin-1), polarity proteins (PKCzeta), Rho regulators and mechanotransducers such as PAK2, Zyxin and YAP.            ZONAB is a DNA and RNA binding factor that it is involved in transcription (e.g. cyclin D1 and PCNA) in the nucleus and translation (e.g. cell cycle inhibitor p21) in the cytosol. Thus, we are also trying to identify new genes regulated. We have identified that expression of fibronectin is regulated by ZONAB. We are evaluating whether the changes in protein expression of fibronectin are due to ZONAB role on transcription or translation, using actinomicin D to inhibit transcription or cyclohexidimide to inhibit translation. Additionally, we are validating new genes identified by cDNA array analysis of endothelial cells with downregulation of ZONAB.            The tight junction localizing protein ZO-1 symptomatically forms a continuous band around the apices of well-differentiated, confluent, polarized epithelial cells in culture. However, under nonconfluent conditions, endogenous ZO-1 can localize to the nucleus in addition to the border of cell-cell contact.            ZONAB manifestation tends to be high in proliferating but low in growth-impeded MDCK cells, implying that high manifestation levels might be a necessity for cell proliferation (Balda & Matter 2000).            ZONAB confines in the nucleus as well as tight junctions in proliferating cells, however, it is not noticeable in the nucleus of nonproliferating high density cells (Balda & Matter 2000), proposing that accumulation of ZONAB in the nucleus might be necessary for efficient proliferation.            ZO-1 quantities are low in proliferating cells and they rise with cell density, and overexpression of ZO-1 hinders accumulation of ZONAB in the nucleus (Balda & Matter 2000); hence, ZO-1 may control proliferation by inhibiting ZONAB from accumulating in the nucleus. Overexpression of ZO-1 in low density cells triggers a redistribution of ZONAB from the nucleus to the cytoplasm and reduced proliferation.            CDK4 is a major regulator of G1/s transition (Sherr 2000; Malumbres & Barbacid 2001). Thus, ZONAB could control proliferation by regulating the process or the localization of CDK4. Since ZONAB binds CDK4, the nuclear pools of the two proteins may diminish in a parallel manner.            Symplekin is combined with ZONAB in the nucleus; hence, it could be argued that Symplekin modulates the transcription activity of ZONAB. Increased expression of Symplekin results in stimulation of the transcriptional suppressor ZONAB. However, it is also noted that Symplekin is absent in endothelial cells (Keon et al. 1996).            ZONAB controls cell cycle entry. ZO-1 overexpression results in a reduction in DNA synthesis, implying that entry into S-phase was distressed.            These experiments will allow understanding the role of ZO-1 and ZONAB in endothelial cells. Depending on the results, we plan to test how these two proteins are involved in endothelial stress conditions such as shear stress and high glucose. Conclusion            The collaboration of ZO-1 with tight junctions can only be significant for the stabilization of ZO-1, as opposed to attaching ZO-1 to the plasma membrane so as to constrain nuclear accumulation of related proteins. This is supported by the opinion that a truncated protein comprising only the HA-tagged SH3 domain accumulated in the Cytosol, but was adequate to decrease proliferation and nuclear accumulation of ZONAB (unpublished data).            ZONAB and ZO-1 control proliferation and the ultimate cell density of MDCK cells. Explanations that ZO-1 accumulates with increasing cell density, and overexpression of ZO-1 in transfected cells lowers the final density proposes a pattern in which ZO-1 serves as a measure for cell density whereby, on reaching the threshold level, provokes growth impediment by cytoplasmic sequestration of ZONAB and the related cell cycle kinase CDK4. It will be essential to control how the ZO-1 or ZONAB pathway associates with the other signaling methods that affect proliferation.            Vascular endothelial stress induces dysfunctions that have been implicated in many diseases such as diabetes and diabetic retinopathy. Therefore, characterization of the role of tight junction molecules in different endothelial cell behavior and functions will help us to understand the molecular mechanisms of disease pathogenesis and these findings may be implicated in prognosis and possibly to develop new treatment strategies. References Balda, MS and Matter, K 2000. The tight junction protein ZO-1 and an interacting transcription factor regulate ErbB-2 expression. EMBO J. 19, pp 2024-2033. Balda MS, Garrett MD and Matter K, 2003. The ZO-1 associated Y-box factor ZONAB regulates epithelial cell proliferation and cell density. J. Cell Biol. 160, pp 423-432. Bargou RC, K Jurchott, C Wagener, S Bergmann, S metzner, K Bommert, MY Mapara, KJ Winzer. M Dietel, B Dorken, and HD Royer, 1997. Nuclear localization and increased levels of transcription factor YB-1 in primary human breast cancers are associated with intrinsic MDR1 gene expression. Nat. Med. 3: pp 447-450. Ciolofan C, Li XB, Olson C, Kamasawa N, Gebhardt BR, Yasumura T, Morita M, Rash JE and Nagy JI, 2006. Association of connexin36 and Zonula occludens-1 with zonula occludens-2 and the transcription factor zonula occludens-1 associated nucleic acid-binding protein at neuronal gap junctions in rodent retina. Neuroscience 140: pp 433-451. Franke WW, P Cowin, C Grund, C Kuhn, HP Kapprell, 1998, The Endothelial Junction: the plaque and its component., in: N. Simionescu, M Simionescu (Eds.), Endothelial cell biology in health and diseases, Plenum publishing corporation, New York. pp 147-166. Giepmans BN and Moolenaar WH, 1998. The gap junction protein connexin43 interacts with the second PDZ domain of the zonal occludens-1 protein. Curr. Biol. 8. Pp 931-934. Gottardi CJ, M Arpin, AS Fanning and D Louvard, 1996. The junction-associated protein, zonular occludens-1, localizes to the nucleus before the maturation and during the remodeling of cell-cell contacts. Proc. Natl. Acad. Sci. USA. 93: pp 10779-10784. Keon BH, S Schafer, C Kuhn, C Grund, WW Franke, Symplekin, a novel type of tight junction plaque protein, J Cell Biol. 134 (1996) 1003-1018.Malumbres M and M Barbacid, 2001. To cycle or not to cycle: a critical decision in cancer. Nat. Rev. Cancer. 1: pp 222-231. Mitic LL and JM Anderson, 1998. 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Listening to the music: Social Problems in Song Mini-Assignment 1 Cory Rossin The song that I have selected with respect to a social problem (Global) is â€Å"March of Death† by Zack De La Rocha lead singer of â€Å"Rage Against Machine†. The intention of this article is to inform the reader of the expressive opinion in reference to the Iraqi war in conjunction with the sociological conflict theory imposed by Karl Marx. â€Å"March of Death implies several messages towards the act of war; consequences and political agendas within the U.S.A. government. â€Å"I won’t toe or defend, instead I curse at murderous men in suits or professionals who act like animals†. This bold statement refers to the new method of colonialism; multi-national corporations who exploit and conquer resources in countries that possess abundant supplies of tangible material and inexpensive labor. Who is in control? Who can repress the American leader, another intriguing statement by the songwriter. Finally the most important message of all, the destructive outcomes of war; thousands of helpless, innocent civilians disfigured and executed by the hands of Goliath himself. The Social problem is evident throughout this song, war and political propaganda a method of achieving the world’s precious energy, oil. In today’s world war is not just a social problem but also a malicious execution of all humanity; in theoretical terms war exhibits all three foundations of a social problem, origin, definition, and treatment (Fleras 2001,10). For conflict theorists war is an inevitable clash between inequalities of wealth, power, privilege, delivered through institutional means within a capitalist system. â€Å"Social problems are inherent in those societies organized around the capitalist principles of profit, competitive individualism, and class conflict† (Fleras 2001,19). â€Å"March of Death† closely reflects the conflict theory by describing religious, ... Free Essays on March Of Madness Free Essays on March Of Madness Listening to the music: Social Problems in Song Mini-Assignment 1 Cory Rossin The song that I have selected with respect to a social problem (Global) is â€Å"March of Death† by Zack De La Rocha lead singer of â€Å"Rage Against Machine†. The intention of this article is to inform the reader of the expressive opinion in reference to the Iraqi war in conjunction with the sociological conflict theory imposed by Karl Marx. â€Å"March of Death implies several messages towards the act of war; consequences and political agendas within the U.S.A. government. â€Å"I won’t toe or defend, instead I curse at murderous men in suits or professionals who act like animals†. This bold statement refers to the new method of colonialism; multi-national corporations who exploit and conquer resources in countries that possess abundant supplies of tangible material and inexpensive labor. Who is in control? Who can repress the American leader, another intriguing statement by the songwriter. Finally the most important message of all, the destructive outcomes of war; thousands of helpless, innocent civilians disfigured and executed by the hands of Goliath himself. The Social problem is evident throughout this song, war and political propaganda a method of achieving the world’s precious energy, oil. In today’s world war is not just a social problem but also a malicious execution of all humanity; in theoretical terms war exhibits all three foundations of a social problem, origin, definition, and treatment (Fleras 2001,10). For conflict theorists war is an inevitable clash between inequalities of wealth, power, privilege, delivered through institutional means within a capitalist system. â€Å"Social problems are inherent in those societies organized around the capitalist principles of profit, competitive individualism, and class conflict† (Fleras 2001,19). â€Å"March of Death† closely reflects the conflict theory by describing religious, ...

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